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After the experimental starvation period the sh were provided with food and their feeding behavior was monitored The two groups were compared to one another, and both were compared to a control group composed of sh that had not been starved (ie would demonstrate a normal hunger level) Given that the sh in both of the experimental groups were hungry they would both be expected to eat more than the members of the control group, and that is exactly what the results showed (Fig 31) The very hungry sh and hungry sh exhibited similarly high levels of hyperphagia at the onset of refeeding but the very hungry sh maintained higher levels of feeding behavior for longer than the hungry sh.

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This suggests that starvation resulted in a strong motivation to feed in both groups, but that as a result of their higher energy de cit the very hungry sh were motivated to feed at higher levels for a longer period If you recall, the original hypotheses were that the very hungry sh would demonstrate either a higher intensity of hyperphagia or a longer duration of hyperphagic behavior than the hungry sh The data clearly support the latter, but why is it that the sh do not seem to do the latter If you want to answer this question I suggest that you skip forward to 6..

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39), one explicitly takes into account the in uence of the liquid vapour interface through the solution surface tension Indeed, consideration of this interface is explicit in the derivation of (639) by including the work required to create this surface in the Gibbs energy summation Since the size of the aerosol particles can range from just a few molecules to particles with radii larger than 100 m, it is important to understand the role the surface effects have on any possible deliquescence process by which small drops are formed [197] There have been few attempts to predict the in uence of curvature on the hygroscopic properties, speci cally in relation to the change in deliquescence patterns There are various complicating features surrounding the point of deliquescence It is the norm to consider the transition from an uncoated dry aerosol to a dissolved salt droplet.

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B. Preparation of cell pellets (a) Grow MSCs in pellet culture according to Protocol 13.7 or 13.8. (b) Centrifuge tube with cell pellet at 3000 g for 10 min at 4 C. (c) Aspirate supernate carefully without destroying the cell pellet. (d) Add 300 l cell lysis solution to each tube. (e) Disintegrate the pellet with a pipette tip by pipetting 20 times up and down. (f) Incubate at room temperature for 48 h. (g) Centrifuge tube at 3000 g for 10 min. (h) Transfer the supernate with a pipette into a clear microcentrifuge tube without destroying the cell pellet. (i) Proceed with DNA Assay or store samples at 20 C until measurement. C. DNA Assay (a) Thaw your samples if frozen and centrifuge at 3000 g for 10 min. (b) Prepare a sample/standard setup table in your lab-book. (c) Calculate the amount of reagents needed as follows: provided in the kit: Reagent A: Reagent B: Reagent C: PicoGreen dsDNA quantitation reagent (in DMSO toxic) 20 TE buffer -DNA standard

Greaser, M L, and J Gergely 1971 Reconstitution of troponin activity from three protein components Journal of Biological Chemistry 246:4226 4233 Guttmann, R P, J S Elce, P D Bell, J C Isbell, and G V Johnson 1997 Oxidation inhibits substrate proteolysis by calpain i but not autolysis Journal of Biological Chemistry 272:2005 2012 Guttmann, R P, and G V Johnson 1998 Oxidative stress inhibits calpain activity in situ Journal of Biological Chemistry 273:13331 13338 Hargreaves, M, and M Thompson (eds) 1999 Biochemistry of Exercise, vol 10 Champaign, Ill: Human Kinetics Harris, S E, E Huff-Lonergan, S M Lonergan, W R Jones, and D Rankins 2001 Antioxidant status affects color stability and tenderness of calcium chloride-injected beef Journal of Animal Science 79:666 677 Hegarty, P V 1970 Differences in bre size of histologically processed pre- and postrigor mouse skeletal muscle Life Sciences 9:443 449.

Another way to add motion to your page is to add a movie. The problem with most movie files is they tend to be considerably larger than animated GIF files. This means they take a lot of time to download although you can do things to get them to start playing before they complete the download and they take up a lot of space on your server. Movies will run in most browsers without any additional plug-ins.

However, several studies have shown that it may be more suitable to consider the transition from a wetted particle to a dissolved droplet [58, 198, 199] Mirabel et al [197] developed a model to predict the prompt deliquescence, and its dependence on particle size, for a generic crystal with an.

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