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With a large water load, the renal response is to produce a large-volume, very dilute urine (osmolality much lower than in blood plasma) In contrast, during a state of dehydration, the urine volume is low and very concentrated (ie, the urine osmolality is much greater than in blood plasma) That the urine osmolality is so variable brings us to a crucial aspect of renal function Terrestrial animals must be able to independently control excretion of salt and water, because their ingestion and loss is not always linked (see Tables 6 1 and 6 3) To excrete water in excess of salt and vice versa (ie, produce a range of urine osmolalities), the kidneys must be able to separate the reabsorption of solute from the reabsorption of water, ie, to separate salt from water How do they do this

1 the mass of the crucible and cover 2 the mass of the original sample and the crucible and cover 3 the mass of the reacted sample and the crucible and cover The last measurement must be done after the sample has cooled to room temperature

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The mass of the sample is calculated by taking the difference between masses 1 and 2 The mass of the substance that combined with the original substance is calculated from the difference between masses 2 and 3 The moles of the substances must be calculated by dividing each mass by the molar mass of the substance The empirical formula is calculated from the simplest ratio of the moles of the elements present

Route Intake Beverage Food Metabolically produced Total Output Insensible loss (skin and lungs) Sweat In feces Urine Total 900 50 100 1500 2550 1200 1000 350 2550 mL/day

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This procedure will allow you to calculate the empirical formula of the substance The experiment is often performed by reacting magnesium metal with atmospheric oxygen to form magnesium oxide

Regardless of hydration state the collective actions of renal tubular segments before the cortical collecting tubule reabsorb more solute than water This leaves a large volume of dilute tubular fluid ( 110 mOsm/kg H2O) entering the limited segment of the cortical collecting tubule If an individual is overhydrated and, therefore, requires maximum water excretion, most of this water simply passes through the collecting-duct system to appear in the urine, with only limited further reabsorption In contrast, when an individual is dehydrated, water excretion should be low The vast majority of this dilute water is reabsorbed, leaving a low volume of concentrated final urine A balance sheet for total body water is given in Table 6 3 These are average values, which are subject to considerable variation The two sources of body water are metabolically produced water, resulting largely from the oxidation of carbohydrates, and ingested water, obtained from liquids and so-called solid food (eg, a rare steak is approximately 70% water) There are several sites from which water is always lost to the external environment: skin, lungs, gastrointestinal tract, and kidneys Menstrual flow and, in lactating women, breast milk constitute two other potential sources of water loss in women The loss of water by evaporation from the cells of the skin and the lining of respiratory passageways is a continuous process, often referred to as insensible loss because people are unaware of its occurrence Additional water evaporates from the skin during the production of sweat Fecal water loss is normally quite small but can be severe in diarrhea Gastrointestinal loss can also be large during severe bouts of vomiting Water reabsorption always occurs in the proximal tubule (65% of the filtered water), descending thin limb of Henle s loop (10%), and collecting-duct system

The amount of water present in a sample is determined by weighing a compound before and after heating The difference in mass is due to the loss of water (See the chapter on Stoichiometry)

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