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Because chloride reabsorption is dependent on sodium reabsorption, the tubular locations that reabsorb chloride and the percentages of filtered chloride reabsorbed by these segments are similar to those for sodium (see Table 6 1) When examining chloride reabsorption, it is helpful to keep in mind the absolute constraint of electroneutrality: any finite volume of fluid reabsorbed must contain equal amounts of anion and cation equivalents Let us do a guesstimate calculation One liter of normal filtrate contains 140 mEq of sodium, and thus must contain about 140 mEq of anions, mainly chloride (110 mEq) and bicarbonate (24 mEq) (We say about because there are other cations [eg, potassium and calcium] and anions [eg, sulfate and phosphate] that must factor into the calculation to achieve an exact balance, but their contributions are much smaller than sodium, chloride, and bicarbonate) If 65% of the filtered sodium is reabsorbed in the proximal tubule, 065 X 140 5 91 mEq of sodium in each liter of filtrate are reabsorbed Therefore, about 91 mEq of some combination of chloride and bicarbonate must also be reabsorbed to accompany this sodium As described in 9, about 90% of the filtered bicarbonate is reabsorbed in the proximal tubule (09 X 24 22) This leaves 91 2 22 5 69 mEq of chloride that must be reabsorbed in the proximal tubule This is more than 60% of the filtered chloride and almost as much as the fractional reabsorption of sodium and water To understand active transcellular chloride reabsorption, it is necessary to recognize that the critical transport step for chloride is from lumen to cell The chloride transport process in the luminal membrane must achieve a high enough intracellular chloride concentration to cause downhill chloride movement out of the cell across the basolateral membrane (of course, the movement of chloride across the basolateral membrane is also promoted by the negative potential within the cell) Thus, luminal membrane chloride transporters serve essentially the same function for chloride that the basolateral membrane Na-K-ATPase pumps do for sodium: They use energy to move chloride uphill from lumen to cell against its electrochemical gradient.

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BasiC renal proCesses for sodium, Chloride, and Water / 81

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The formula of a compound is determined by using the mass of the original substance, usually a metal, and the mass of a compound of that substance, usually an oxide (See the chapter on Stoichiometry)

Figure 6 1 Major pathways for reabsorption of sodium, chloride, and water in the proximal tubule The entire proximal tubule is the major site for reabsorption of salt and water Sodium entry is coupled with the secretion or uptake of a variety of substances, the major one being secreted hydrogen ions via the NHE-3 antiporter These hydrogen ions combine with filtered bicarbonate and secreted organic base (see text and 9 for further explanation) Additional sodium enters in symport with glucose, amino acids, and phosphate Sodium is transported to the interstitium mostly via the basolateral Na-K-ATPase, and also in symport with bicarbonate (The coupling between sodium and bicarbonate is described fully in 9) Chloride that enters in antiport with organic base leaves mostly via channels In addition, a substantial amount of chloride is reabsorbed paracellularly Water moves both paracellularly and intracellularly via aquaporins ATP, adenosine triphosphate

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(Continued )

According to the luminal membrane depicted in Figure 6 1, the major routes are (1) paracellular absorption and (2) a complicated parallel set of Na-H and Cl-base antiporters (described later) These mechanisms are dependent on sodium movement across the membrane and are, therefore, linked to sodium reabsorption

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